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| 13. |  | MEDRI, I. M.; MOURÃO, G.; MARINHO FILHO, J. Morfometria de tatu-peba, Euphractus sexcintus (Linnaeus, 1758), no Pantanal da Nhecolândia, MS. Edentata, n.8-10, p. 35-40, 2009.| Biblioteca(s): Embrapa Pantanal. |
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| 14. | | MEDRI, I. M.; MARTINS, J. R.; DOYLE, R. L.; MOURAO, G. de M.; MARINHO-FILHO, J. Ticks (Acari: Ixodidae) from yellow armadilho, Euphractus sexcinctus (Cingulata: Dasypodidae), in Brazil´s Neotropical Entomology, v.39, n.5, p.823-825, out. 2010. Scientific note.| Biblioteca(s): Embrapa Pantanal. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
16/07/2010 |
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Data da última atualização: |
16/07/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
PEDRINI, S. C. B.; ROSA, P. S.; MEDRI, I. M.; MOURAO, G. de M.; BAGAGLI, E.; LOPES, C. A. de M. |
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Afiliação: |
SILVIA CRISTINA BARBOZA PEDRINI, INSTITUTO LAURO DE SOUZA LIMA; PATRÍCIA SAMMARCO ROSA, INSTITUTO LAURO DE SOUZA LIMA; ÍSIS MERI MEDRI; GUILHERME DE MIRANDA MOURAO, CPAP; EDUARDO BAGAGLI, UNESP; CARLOS ALBERTO DE MAGALHÃES LOPES, UNESP. |
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Título: |
Search for Mycobacterium leprae in wild mammals. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Brazilian Journal of Infectious Diseases, v. 14, n.1, p. 47-53, 2010. |
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Idioma: |
Português |
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Conteúdo: |
Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the
experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed
samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state. MenosLeprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the
experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed
samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected f... Mostrar Tudo |
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Palavras-Chave: |
Eco-epidemiology; Euphractus sexcinctus; Wild mammals. |
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Thesagro: |
Dasypus Novemcinctus. |
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Thesaurus NAL: |
Cabassous tatouay; Mycobacterium leprae. |
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Categoria do assunto: |
-- |
|
Marc: |
LEADER 02624naa a2200253 a 4500
001 1858047
005 2010-07-16
008 2010 bl --- 0-- u #d
100 1 $aPEDRINI, S. C. B.
245 $aSearch for Mycobacterium leprae in wild mammals.
260 $c2010
520 $aLeprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus sexcinctus, Cabassous unicinctus, and C. tatouay armadillos from the Middle Western region of the state of São Paulo and from the experimental station of Embrapa Pantanal, located in Pantanal da Nhecolândia of Mato Grosso do Sul state. Also, the molecular analysis of 19 samples from internal organs of other road killed species of wild animals, such as Nasua nasua (ring-tailed coati), Procyon cancrivoros (hand-skinned), Cerdocyon thous (dog-pity-bush), Cavia aperea (restless cavy), Didelphis albiventris (skunk), Sphigurrus spinosus (hedgehog), and Gallictis vittata (ferret) showed PCR negative data. None of the 157 analyzed samples had shown natural mycobacterial infection. Only the armadillo inoculated with material collected from untreated multibacillary leprosy patient presented PCR positive and its genomic sequencing revealed 100% identity with M. leprae. According to these preliminary studies, based on the used methodology, it is possible to conclude that wild mammals seem not to play an important role in the epidemiology of leprosy in the Middle Western region of the São Paulo state and in the Pantanal of Mato Grosso do Sul state.
650 $aCabassous tatouay
650 $aMycobacterium leprae
650 $aDasypus Novemcinctus
653 $aEco-epidemiology
653 $aEuphractus sexcinctus
653 $aWild mammals
700 1 $aROSA, P. S.
700 1 $aMEDRI, I. M.
700 1 $aMOURAO, G. de M.
700 1 $aBAGAGLI, E.
700 1 $aLOPES, C. A. de M.
773 $tBrazilian Journal of Infectious Diseases$gv. 14, n.1, p. 47-53, 2010.
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